Coding
Part:BBa_K5374023:Design
Designed by: Langning Xu Group: iGEM24_TestDaily-I-Beijing (2024-09-24)
VWF-A Domain-EGFP (von Willebrand Factor A Domain). A domain known for mediating collagen binding, p
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 112
Illegal PstI site found at 875
Illegal PstI site found at 1282 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1330
Illegal PstI site found at 112
Illegal PstI site found at 875
Illegal PstI site found at 1282 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 849
Illegal BamHI site found at 1017 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 112
Illegal PstI site found at 875
Illegal PstI site found at 1282 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 112
Illegal PstI site found at 875
Illegal PstI site found at 1282 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1973
Design Notes
The sequence was optimized for EGFP fusion, ensuring the domain’s collagen-binding function while maintaining fluorescence for activity evaluation.
Source
VWF-A domain from von Willebrand factor, with EGFP from BBa_K1911005.